Epidemiological study with E3N women and their families

The E3N-Generation study is interested in the influence of the “exposome” - the environment in the broad sense and contemporary lifestyle on health, among people of the same family, over three generations.

Key elements

  • Generalist cohort - over three generations
  • 140,000 participants
  • Since 1990

Objectives of the cohorte

The E3N-Generations study relies on a community of families, a family cohort that will eventually have 200,000 participants.

It extends the E3N study which has been actively following since 1990 (30 years!) 100,000 women, by inviting their children, the fathers of those children and their grandchildren to participate in turn.

E3N-E4N is one of only two epidemiological studies in the world of this magnitude bringing together families across three generations.

The members of the same family have genes, habits and living places in common. This vast community of families is a powerful research tool for unraveling what is genetic, lifestyle or environmental in our health.

The study will provide a better understanding of what exposes us to chronic diseases or protects us from them, by comparing the evolution of the health of different groups within the cohort. Researchers are interested in cancers, cardiovascular diseases, inflammatory bowel diseases, diabetes, asthma, Parkinson's disease, depression, anxiety, endometriosis, but also aging well.

The study is involved with other cohorts in research consortia to analyze such topics on a larger scale. That is the case of the European EPIC consortium on cancer (more than 500,000 subjects) and the French SAPRIS study on Covid19 (139,000 participants from 5 cohorts).

This research will make it possible to drive future public health policies and refine prevention messages.

Recent publications

  • MacDonald CJ, El Fatouhi D, Anne-Laure Madika AL, Fagherazzi G, Kurth T, Severi G, Boutron-Ruault MC. Association of Migraine With Incident Hypertension After Menopause. A Longitudinal Cohort Study. Neurology Jul 2021, 97 (1) e34-e41
  • Dietary exposure to brominated flame retardants and risk of type 2 diabetes in the French E3N cohort. Ongono JS, Dow C, Gambaretti J, Severi G, Boutron-Ruault MC, Bonnet F, Fagherazzi G, Mancini FR. Environ Int. 2019 Feb;123:54-60. doi:10.1016/j.envint.2018.11.040.
  • Stringhini S, Carmeli C, Jokela M, Avendaño M, Muennig P, Guida F, Ricceri F, d'Errico A, Barros H, Bochud M, Chadeau-Hyam M, Clavel-Chapelon F, et al; LIFEPATH consortium. Socioeconomic status and the 25 × 25 risk factors as determinants of premature mortality: a multicohort study and meta-analysis of 1·7 million men and women. Lancet. 2017 Mar 25;389(10075):1229-1237.
  • Beelen R, Raaschou-Nielsen O, Stafoggia M, Andersen ZJ, Weinmayr G, Hoffmann B, Wolf K, Samoli E, Fischer P, Nieuwenhuijsen M, Vineis P, Xun WW, Katsouyanni K, Dimakopoulou K, Oudin A, Forsberg B, Modig L, Havulinna AS, Lanki T, Turunen A, Oftedal B, Nystad W, Nafstad P, De Faire U, Pedersen NL, Ostenson CG, Fratiglioni L, Penell J, Korek M, Pershagen G, Eriksen KT, Overvad K, Ellermann T, Eeftens M, Peeters PH, Meliefste K, Wang M, Bueno-de-Mesquita B, Sugiri D, Krämer U, Heinrich J, de Hoogh K, Key T, Peters A, Hampel R, Concin H, Nagel G, Ineichen A, Schaffner E, Probst-Hensch N, Künzli N, Schindler C, Schikowski T, Adam M, Phuleria H, Vilier A, Clavel-Chapelon F et al. Effects of long-term exposure to air pollution on natural- cause mortality: an analysis of 22 European cohorts within the multicentre ESCAPE project. Lancet. 2014 Mar 1;383(9919):785-95.
  • Stringhini S, Carmeli C, Jokela M, Avendaño M, McCrory C, d'Errico A, Bochud M, Barros H, Costa G, Chadeau- Hyam M, Delpierre C, Gandini M, Fraga S, Goldberg M, Giles GG, Lassale C, Kenny RA, Kelly-Irving M, Paccaud F, Layte R, Muennig P, Marmot MG, Ribeiro AI, Severi G, et al. LIFEPATH Consortium. Socioeconomic status, non-communicable disease risk factors, and walking speed in older adults: multi-cohort population based study. BMJ. 2018 Mar 23;360:k1046.

Management and governance

The E3N-Generation study is led by Gianluca Severi, research director at the National Institute of Health and Medical Research (Inserm U1018-CESP). Its governance is ensured by the founding partners: Inserm, the University of Paris-Saclay, the Gustave Roussy Institute, the National League Against Cancer and the National Education General Mutual Benefit Society (MGEN).

Terms and conditions of accessing cohort data

  • Requests for access to data and biological samples from the E3N-E4N cohort for research projects should be sent to: contact@e3n.fr.
  • Assessed by the cohort's access committee according to the endpoints of scientific quality, relevance, quality of the team or teams leading the project, and the relationship with other ongoing projects.
  • Access to data is subject to obtaining the necessary funding and authorizations from the authorities concerned.

Detailed information